ricevimento lunedì ore 15,30 presso Sezione Scienze Biochimiche
Dipartimento di Scienze Biomediche Sperimentali e Cliniche
CURRICULUM SCIENTIFICO della Prof.ssa Donatella Degl'Innocenti.L’attività scientifica è iniziata con la purificazione da tessuti di acilfosfatasi e la produzione di anticorpi policlonali per l’allestimento di cromatografia di affinità e dosaggi immunochimici delle proteine studiate.In seguito ha effettuato la caratterrizzazione immunologica di varie acilfosfatasi e l’identificazione dei corrispondenti epitopi e prodotto anticorpi monoclonali verso la forma umana. La coltivazione di cellule ha permesso di effettuare studi di differenziazione sia su cellule umane che murine correlando tali processi con i livelli di acilfosfatasi. In seguito mediante le tecniche di biologia molecolare ha clonato vari geni ed espresso le relative proteine. Inoltre mediante RT-PCR ha effettuato studi di espressione di acilfosfatasi. Ulteriori avanzamenti nelle tecniche immunologiche hanno permesso alla D. Degl’Innoceneti, mediante screening di library fagiche, la selezione e produzione di specifici anticorpi ricombinati a singola catena (scFv) diretti contro epitopi conformazionali di acilfosfatasi e hVH contro peptidi. Ha svolto studi su estratti di alghe e piante marine.
La prof. Degl’Innocenti dal 1996 è responsabile di fondi di ricerca d’Ateneo (ex 60%), fondi Ente Cassa di Risparmio di Firenze e di fondi PRIN ed è sempre stata inserita in progetti PRIN finanziati.
Legenda
Donatella Degl’Innocenti, Associate Professor in Biochemistry, University of Florence .
1983 was graduated in Biological Sciences, summa cum laude (110 Lode/110), at the University of Florence on October 17th, with a thesis “ Molecular characterization of acylphosphatase from rabbit muscle” 1985-89 doctoral research fellow at the Department of Biochemistry, University of Florence, Her scientific activity ha been dedicated to the study of several type of acylphosphatase enzymes and their immunological characterization. 1991-2001 was researcher at the Faculty of Pharmacy, University of Florence, working on cellular differentiation and antibody production. 1990 PhD in Biochemistry, thesis “Immunological characterization of acylphosphatases” 2001 won a public competition for Associate Professor in Biochemistry (BIO10) at the Faculty of Pharmacy, University of Florence
In 2005 she was Chair of the graduated school in “Pharmaceutic Biotechnologies” of the Faculty of Pharmacy, University of Florence, confirmed also for 2009-2013
Scientific Activity
Her activity was dedicated to cellular biochemistry of differentiation and molecular biology of antibodies.Her scientific work stated with acylphosphatase purification from different tissues and polyclonal antibodies selection and purification to perform affinity chromatography and immunochemical assays against deffering acylphosphatases. She carried out the immunological characterization of some acylphosphatases and theirs corresponding epitopes and selected monoclonal antibodies against the human acylphosphatase. Using cell cultures she carried out studies on differentiation of human and murine cells to correlate these process with the intracellular level of acylphosphatase. Later on by molecular biology techniques she performed gene cloning experiments and expressed their encoded proteins. Moreover by RT-PCR she performed studies on acylphosphatase expression. More recently, the improvements of the immunological techniques allowed her to selected and produced specific recombinant single chain fragments (scFvs) against specific conformational epitopes of acylphosphatase, and amyloid aggregates.